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Image Search Results
Journal: Asian Journal of Pharmaceutical Sciences
Article Title: Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors
doi: 10.1016/j.ajps.2022.07.002
Figure Lengend Snippet: In vivo immune responses assessment. (A) Flow cytometry assay of CD80 + or CD86 + gated on CD11c + in the TDLNs of mice. (B) Flow cytometry assay illustrated the percent of CD8a + T cells and CD4 + T cells gated on CD3 + T cells in splenocytes of mice. (C-D) Quantitative percentages of CD80 + or CD86 + expressions gated on CD11c + according to (A). (E) Quantitative percentages of CD8a + T cells and CD4 + T cells in splenocytes according to (B). (F-H) Quantitative analysis of the immune-associated cytokines in serum, as determined by ELISA (IL-2, TNF-α and IFN-γ). ( n = 3, mean ± SD, * P < 0.05, ** P < 0.01 and ## P < 0.0001).
Article Snippet: Fluorochrome-labeled anti-mouse monoclonal antibodies (CD11c-allophycocyanin (APC), CD80-fluorescein isothiocyanate (FITC), CD86-phycoerythrin (PE), CD3-FITC,
Techniques: In Vivo, Flow Cytometry, Enzyme-linked Immunosorbent Assay
Journal: bioRxiv
Article Title: Assessing a Role for Sarcospan in Immune Function
doi: 10.1101/2024.03.31.587501
Figure Lengend Snippet: Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and CD8+ T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Article Snippet: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend, San Diego, CA; clone 6D5), CD3-AlexaFluor488 (BioLegend, clone 17A2), CD4-violetFluor450 (Tonbo Biosciences, clone GK1.5),
Techniques: Flow Cytometry, Two Tailed Test, IF-P
Journal: Journal of Extracellular Vesicles
Article Title: A multiantigenic antibacterial nanovaccine utilizing hybrid membrane vesicles for combating Pseudomonas aeruginosa infections
doi: 10.1002/jev2.12524
Figure Lengend Snippet: In vivo immune responses activated by AuNP@HMV@SPs. (a) Schematic illustration of experimental design to evaluate the in vivo immune responses activated by AuNP@HMV@SPs (OMVs: 2 µg/mL, SP: 1.5 µg/mL) via subcutaneous injection. (b) Percentage of CD80 + and CD86 + cells (gated on CD11c + cells) in the lymph nodes collected on day 21 ( n = 4). (c) Percentage of GL7 + cells (gated on CD19 + IgG − cells) in the spleen collected on day 21 ( n = 5). (d) Anti‐ P. aeruginosa specific antibody IgG titers in serum collected on day 21 ( n = 5). (e) CD3 + T cells, (f) CD3 + CD4 + T cells, and (g) CD3 + CD8 + T cells in the spleen collected on day 21 ( n = 5). Naive mice without immunization were used as control. Statistical analysis was conducted using the student's t ‐test or One‐way ANOVA with Tukey's post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001 and ns representing non‐significance.
Article Snippet: FITC‐conjugated anti‐mouse CD11c, PE‐conjugated anti‐mouse CD80, APC‐conjugated anti‐mouse CD86, APC‐conjugated anti‐mouse CD3, PE‐conjugated
Techniques: In Vivo, Injection, Control